FASAY, cell cycle regulation proteins and oncogene activation in UCMSC.

<p>A. FASAY results analyzing the <i>TP53</i> gene isolated either from UCMSC or <i>p53</i>-mutated cells issued from a Li-Fraumeni patient. White yeast colonies are indicative of a wild-type <i>p53</i> allele while mutated <i>p53</i> induces red colony formation. B. Representative interphase FISH analysis of the TP53 locus confirming the presence of both gene alleles in the 17p13.1 region. C. Western blot analysis of p21, p53, p16, pRb and c-Myc proteins in UCMSC at different passages (P3, P9 and P18). Cervix cancer HeLa cells were used as a control. p21 was not detected in HeLa cells, while both c-Myc and phosphorylated pRb were up-regulated. The high level of p16 protein in HeLa cells was previously reported <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071374#pone.0071374-Soucek1" target="_blank">[56]</a> but does not impair cell growth because pRb is inhibited by E7 papillomavirus protein in that cell line. The c-Myc protein of HeLa cells is of higher molecular weight because of the insertion of a mitochondrial DNA fragment in the <i>c-Myc</i> locus <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071374#pone.0071374-Shay1" target="_blank">[57]</a>.