Effects of ANXA2 on the migration and invasion of HCC cells co-cultured with HPF-1 cells.

<p>Twenty-four hours after transfection with si-ANXA2 or snc-RNA, HCC cells were co-cultured with an equal number of HPF-1 cells in the upper chamber, which were not coated with Matrigel in the <i>in vitro</i> migration assay (A) but were coated in the <i>in vitro</i> invasion assay (B). Eight (migration assay) or twenty-four (invasion assay) hours later, the cells migrating/invading through the filter were stained and counted. Left, representative images showing the density of cells on the filter. Right, quantitative analyses of the cells migrating/invading through the filter in three independent experiments. Columns, mean; bars, SD. <i>p<0.001</i> vs. corresponding snc-RNA-transfected cells (one-way ANOVA). (C) Silencing ANXA2 in HCC cells inhibited the cellular secretion of MMP-2 by HPF-1 cells cultured in supernatant collected from si-ANXA2-transfected HCC cells. Serum-free conditioned medium collected from si-ANXA2- or snc-RNA-transfected HCC cells was added to HPF-1 cells. Fifteen hours later, the conditioned medium was collected and analyzed using gelatin zymography. Left, representative image. Right, gray scale analysis of at least three independent experiments. Columns, mean; bars, SD. <i>p<0.01</i> vs. corresponding snc-RNA-transfected cells (one-way ANOVA).