Effects of MTX with or without fish oil (FO) and/or genistein (Gen) supplementation on growth plate, primary and secondary spongiosa.

<p>Paraffin sections of the tibial metaphysis region (PS = Primary spongiosa, SS = Secondary spongiosa, which are separated by a dashed line) of (<b>A</b>) a normal rat, (<b>B</b>) a MTX+H₂O treated rat showing reduced height of primary spongiosa and. metaphyseal bone volume, (<b>C</b>) a MTX+FO treated rat, and (<b>D</b>) a MTX+Gen treated rat. (<b>E</b>) Growth plate total height (µm). (<b>F</b>) Primary spongiosa height (µm). (<b>G</b>) Secondary spongiosa BV/TV (%). (<b>H</b>) Secondary spongiosa osteoblast/mm² trabecular bone area. Values are means ± SEM; n = 7–8 for all groups. Labelled means without a common letter differ (P<0.05).