Analysis of the proteins expressed in <i>Escherichia coli</i> TOP10 cells following purification on a 15% denaturing polyacrylamide gel.

<p>Lanes M, protein standards; 1, before induction with 1 mM IPTG; 2, after cells were induced with IPTG and grown at 37°C for 5 h; 3, recombinant protein purified under native conditions with the ProBond™ purification system. Arrows designate the positions of the proteins on the gels.