The MVA13.5L promoter.

<p>(A) The MVA sequence 250 nucleotides (nt) upstream of the MVA13.5L open reading frame (ORF) with a ‘left’ orientation is shown (GenBank accession number AY603355). The ATG encoding for the methionine (Met) within the MVA13.5L ORF is shown in bold. The arrow indicates the direction of transcription for the MVA13.5L gene. The two transcriptional start sites (TSS) for the MVA13.5L gene are indicated by black arrow heads. TSS1 correspond to nt 9 978 and TSS2 corresponds to nt 10 058 in the MVA genome. The two 44 nt repeats, denoted R1 and R2, are shown as grey sequences. The 36 nt spacer region between R1 and R2, denoted SP, is shown as sequences in italic. The sequences used to construct the PrMVA13.5-long promoter (9 965–10 088 nt) are underlined with a solid line. Sequences used to construct the PrMVA13.5-short promoter (9 965-10 019 nt) are underlined with a dashed line. (B) The features of the native PrMVA13.5-long and PrMVA13.5-short promoter are depicted graphically. The PrMVA13.5-long consists of the two repeated motifs (R1 and R2) shown as grey boxes separated by the spacer region (SP). The PrMVA13.5-short consists of only the R2 motif and 11 nt of the spacer region (SP). The TSS1 is shown as a black arrowhead. The promoters were used to generate an OVA expression cassette introduced into the MVA genome at IGR 44/45. The position of the promoter sequences relative to the start (ATG) of the OVA open reading frame (ORF) is shown. Arrows indicate the direction of transcription.