Association of an Insertion Event to a Specific Line by 2D-PCR Pooling.

<p>A. Workflow of the 2D-PCR pooling B. An example of the pooling design for 16 plants. Each plant genomic DNA is pooled in a unique set combination. The plants encompass by the colored rectangle associate to the pool of the same color. C. Data analysis to identify the positive line. All bands on a given gel correspond to the same amplification product in different pools.