Metabolism related changes during HFD transition.

<p>Mice in CLAMS were switched from ND to HFD at the beginning of a light cycle. (A) Light cycle and (B) dark cycle calories consumed per 12 hr cycle (N = 7). (C) Light cycle and (D) dark cycle respiratory exchange ratio (RER; VC0₂:VO₂). (E) Light and (F) dark cycle average movement (x-ambulations) per 12 hour cycle. Data are mean ± SEM. Statistics: Repeated measures one-way ANOVA with Tukey’s multiple comparison test, p<0.05. ND (white bars), HFD (black bars). Average CLAMS measurements per cycle are total (A–B) or means (C–F) of measurements taken every 20 minutes with 1 mouse/CLAMS cage, N = 8 unless otherwise noted. Light cycle = 07∶00–19∶00, dark cycle = 19∶00–07∶00.