CytoSNP interrogation of human Pluripotent Stem Cell lines.

<p>KaryoStudio Detected Regions for NHDF-OX1 (parental fibroblasts) and derived hiPSC lines iPS-OX1-18, iPS-OX1-19 and iPS-OX1-23. The chromosomes which contained regions detected by KaryoStudio as deviating from expected (indicated in ‘Found Region’ column by green for amplification, orange for deletion) are shown, as are the X and Y chromosomes (which, being single copy, are Called despite being the expected copy number). Where an amplification has been detected, the SNP B allele frequency can be seen to show a corresponding small triploid region and the Smoothed Log R Ratio shows a small spike. Chromosomes that are not shown here were those (the majority) that had no regions detected by KaryoStudio as deviating from expected, and so are considered karyotypically normal. Note that the small changes detected here are well below the level of detection in other karyotype methods. The genes that these affect are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071098#pone-0071098-t001" target="_blank">Table 1</a>.