HCA1 receptor involvement in the lactate sensitivity.

<p>(a) Confocal images showing immunostaining for NeuN (green), HCA1 (red) and the merged image in mouse primary cortical neurons. Scale bar, 20 µm. (b) Representative Western blot showing that HCA1 is expressed in mouse primary cortical neuronal cultures. Each track represents one independent cultured dish of mouse primary cortical neurons (c) Comparison of lactate effect on calcium spiking frequency in cells incubated or not with pertussis toxin (PTX). PTX incubation strongly reduced the effects of lactate on neuronal activity. Data are obtained from 8 experiments and 61 cells for non-treated group and 8 experiments and 62 cells for PTX treated group.