Concentration–response Functions for 8 Different Sweeteners in a Single Test Session.

<p>Eight sweeteners (SUC = sucrose, REB A = rebaudioside A, ACE K = acesulfame potassium, SCR = sucralose, SAC = saccharine, STEV = stevioside, GLY = L-glycine, and SC45647) were tested for both taste quality and palatability across a range of concentration. Positive control: 100 mM SUC = sucrose; Negative controls: 10 mM CIT = citrate, 1 mM QUI = quinine, water and 100 mM NaCl. Upper panels shows graphs of sweet taste quality plotted as percent of responses made on the sucrose lever. Lower panel shows concentration–response functions for palatability, plotted as mean licks/trial, obtained in the same experiment. Each concentration of test article was dispensed into a single well of a 96-well plate. Data are plotted as mean of responses to contents from a single well per concentration of test article and 6-7 wells for controls per rat, averaged across 4 rats. Error bars are SEM. Data are representative of 3 equivalent experiments. See Figure S5 for plate configuration corresponding to the experiment illustrated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072391#pone-0072391-g006" target="_blank">Figure 6</a>.