Guanine and cytosine (GC) content, CpG site, and hotspot mutations in <i>TP53</i>.

<p>Frequencies of <i>TP53</i> mutations and their locations with GC content and CpG density. Most mutations were located in DNA binding domain (A). Hotspot mutations (five or more frequencies in 301 patients) were more likely to occur at CpG sites (<i>p</i><0.001, chi-square test) (B). Functional severity scores of hotspot mutant p53 proteins (n=74) were much higher than those of p53 mutant proteins that occurred outside of the hotspots (n=111) (<i>p</i>=0.0074, Wilcoxon rank sum test) (C).