HPV16 E6 suppresses the ING4 mediated p53 transcriptional activity and apoptosis.

<p>(C) U2OS cells were respectively transfected with siRNA Luciferase or siRNA E6AP, with ING4 or ING4+E6 using the Lipofectamin 2000. Cells were collected at 36 h posttransfection after a 12-h serum starvation and fixed. Levels of cells undergoing apoptosis (sub-G1 phase) in individual PI-stained samples were analyzed by flow cytometry, and the data were analyzed by FlowJo software. The bar diagram represents the mean of three independent experiments. Western blot showing the protein level of E6AP in the lentivirus-mediated E6AP of control knockdown cell lines. GAPDH was used as the loading control.