Representative frequencies of phenotypes, CFCs, and MRUs in E18.5 fetal and adult mammary tissue.

<p>(A) Representative gating and final FACS profiles of viable subsets of single cells isolated from dissociated E18.5 mammary glands after exclusion of hematopoietic (CD45⁺ and Ter119⁺) and endothelial (CD31⁺) cells. Embedded images are negative controls. (B) Frequency of total cells, CFCs, and MRUs measured in the indicated subsets and in suspended but not further separated fetal mammary (EpCAM⁺) cells. (C) Representative gating and final FACS profiles of viable subsets of single cells isolated from dissociated 8–12-wk-old adult mammary glands after exclusion of hematopoietic (CD45⁺ and Ter119⁺), endothelial (CD31⁺), and stromal (BP-1⁺) cells. A fluorescence-minus-one control was used to set the gate used to identify CD61⁺ cells. (D) Frequency of total cells, CFCs, and MRUs measured in the indicated subsets and in unseparated suspensions of adult mammary epithelial (EpCAM⁺) cells.