Verification of c-kit expression in aptamer sorted bone marrow cells.

<p>Mouse bone marrow cells were double labeled with anti-CD45 and Kit-129 aptamer. Kit-129 positive and negative populations were sorted (A) and qPCR performed to analyze c-kit expression within each population. The CT value for the c-kit positive population was 27.3 while c-kit mRNA was undetectable even after 40 cycles for the c-kit negative population. ActinB was used as an internal control and c-kit expression data was normalized to ActinB. (B) Products from qPCR analysis run on a 2% agarose gel.