ZO-1- α5-integrin complex formation is required for cytokinesis.

<p>(A) Time lapse imaging (1 frame/10 min for 4 h) of control and α5-integrin silenced NCI-H460 cells. (B) Micrograph of α5-integrin silenced NCI-H460 cells stained as indicated. (C) NCI-H460 cell in cytokinesis stained as indicated. (D) α5-integrin silenced NCI-H460 cells expressing siRNA resistant WT or PPAA (unable to bind ZO-1) α5-integrin in cytokinesis stained as indicated. All cells were plated on fibronectin and dapi was used to visualize nuclear morphology. (E) TIRF (red) and epifluorescence (green) microscopy of ZO-1 or control silenced NCI-H460 cells stained for active β1-integrin during cytokinesis. All cells were growing on 5 µg/ml fibronectin. All arrows indicate the cleavage furrow. All scale bars are 10 µm.