Addition of FGF4 diminishes DAG-induced expression of Runx2 and osterix in mESCs.

<p>Cells were incubated in the presence or absence of DAG and 50 ng/ml FGF4 for 24 h, and expression patterns of bone-specific genes were analyzed by real-time RT-PCR. **p<0.01 and ***p<0.001 vs. untreated control cells. ^#p<0.05 vs. cells treated with DAG only.