Exogenous FGF4 decreases osteogenic differentiation of mESCs.

<p>(A) mESCs were incubated in the presence of DAG and/or 50 ng/ml FGF4. After 5 days of incubation, DNA synthesis (left panel) and cell proliferation (right panel) rates were determined. At the same time, cells were processed for Alizarin red staining (B) and absorbance of the dye (C) was also determined at 560 nm. (D) Runx2 protein in mESCs cultured with DAG and/or 50 ng/ml FGF4 for 5 days was analyzed by flow cytometry. (E) ALP activity in the cells was measured after 3 and 5 days of differentiation. *p<0.05, **p<0.01 and ***p<0.001 vs. untreated control cells. ^#p<0.05 vs. cells treated with DAG only.