The combinative effects of 2-ME and As₂O₃ on cell viability and the level of cleaved caspase-3 and 7 in UC cells.

<p>(A) NTUB1 and (B) T24 cells were incubated in the presence of 2-ME (0.5 and 1 µM) and different concentration of As₂O₃ (0.75 to 10 µM) individually or in combination. Cell viability was measured by MTT assay. Quantitative analysis of cell viability are presented as means ± SD of three independents experiments. * p<0.05 is interpreted to be significant as compared with As₂O₃ treatment alone. The total cell lysates were harvested and analyzed by Western blot with specific antibodies against cleaved caspase-3 and 7 after treatment of 2-ME (1 µM), As₂O₃ (1 µM) and in combination. Results shown are representative of at least three independent experiments.