Crystal structure of Grb14^RA-PH-H-Ras and comparison to other RA-PH structures.

<p>(A) Ribbon diagram and molecular surface representation of the Grb14^RA-PH-H-Ras complex. The Grb14 RA domain is colored green, with β2 colored dark green, the PH domain is colored orange, with the phosphoinositide-binding β1-β2 loop colored magenta, and the RA-PH linker is colored gray. H-Ras is colored cyan, with switch 1 (residues 32-38) colored purple and switch 2 (residues 59-67) colored magenta. GTP is shown in stick representation with carbon atoms colored yellow, oxygen atoms red, nitrogen atoms blue, and phosphorus atoms black. The Mg²⁺ ion is colored brown. Select secondary-structure elements are labeled, along with the N- and C-termini. The view on the right is rotated by 90° as indicated. (B) Stereo diagram of a superposition of the structures (Cα traces) of Grb14^RA-PH, Grb10^RA-PH (PDB code 3HK0) [12], and RIAM^RA-PH (PDB code 3TCA) [17], in the same orientation as in the left panel of (A). Grb14^RA-PH is colored by domain as in (A), Grb10^RA-PH is colored blue (all) and RIAM^RA-PH is colored magenta (all). The N- and C-termini are labeled.