Freshly isolated mitochondria were assessed for changes in membrane potential by JC-1 red and green fluorescence assay and Cytochrome C release by titerzyme enzyme immuno-metric assay kit.

<p>(A) Changes in mitochondria membrane potential by JC-1 in which the ratio of red to green determines the rate of membrane potential decay and cells dead. (B) Cytochrome c release. Results are expressed as mean values ± SD; (n = 6). Statistical significance (p<0.01) is indicated by asterisk compared with controls or compared with EtOH (double stars).