The effect of whole blood stimulation with different cytokines or CCL5 on monocyte subsets.

<p>Whole blood samples (n = 7 animals) were incubated with the cytokines IFNγ, IL-4/IL-13, TNF-α or IL-1β or the chemokine CCL5 for 4 h. Leukocytes were stained with monoclonal antibodies to CD172a, CD14 and CD16. After gating on vital (PI-negative) MNC, agate was made on CD172a-positive cells. CD172a-positive cells were presented in CD14 and CD16 dot plot. Mean fluorescence intensity of CD16 (A) and the percentage (B) of the three monocyte subsets was calculated and presented as mean ± SEM. Differences between groups were calculated using the one-way ANOVA and were considered significant (*) if p<0.05.