Inhibition of the PI3K/Akt pathway increases stretch-induced WPB exocytosis and leukocyte adhesion while inhibition of PKA has the opposite effect.

<p>(A) vWF release from HUVECs pretreated with H89 (50 µM), LY294002 (50 µM) or vehicle (DMSO) in response to stretch. (B) vWF release from HUVECs expressing scrambled (Scr), PKA-targeting (PKA KD), or Akt1/2-targeting (Akt KD) shRNA under continuous stretch. (C) <i>Left</i>: HL-60 cell adhesion to HUVEC monolayers after stretch, pretreated with H89 (50 µM), LY294002 (50 µM) or vehicle (DMSO). <i>Right</i>: quantitative analysis of HL-60 adhesion. (D) <i>Left</i>: HL-60 cell adhesion to HUVECs expressing scrambled (Scr), PKA-targeting (PKA KD), or Akt1/2-targeting (Akt KD) shRNA after stretch. <i>Right</i>: quantitative analysis of HL-60 adhesion. Results are representative of 3 individual experiments and expressed as mean ± S.D. (n = 4), **p<0.01.