The protein expression of aquaporin-1a1 is reduced by morpholino knockdown.

<p>(A) A representative western blot showing the protein expression of aquaporin-1a1 (AQP1a1) in lysates of 4 dpf shams and AQP1a1 morphants (AQP1a1 MO) is reduced following morpholino knockdown. (B) Subsequent quantitative analysis revealed that the protein expression level of AQP1a1 in the morphants was significantly than shams (Student's <i>t</i>-test; <i>p</i><0.05). Values are means ± SEM (<i>n</i> = 4). Fluorescent immunohistochemistry and confocal microscopy revealed the expression of (C) AQP1a1 (green; arrowhead) and (D) Na⁺/K⁺-ATPase (NKA; red) on the skin of yolk sac in the shams. AQP1a1 was also expressed in the red blood cells (Figure C; arrow). Figure (E) is a merged image of (C) and (D). (F) The expression of AQP1a1 was virtually absent in the morphants following the AQP1a1 knockdown. Figure (G) shows the expression of NKA, and Figure (H) is a merged images of (F) and (G). Scale bar = 50 μm.