Co-culture of human primary hepatocytes and 201B7 in hepatocyte selection medium (HSM).

<p>After establishment of a co-culture of 201B7 and human primary hepatocytes (A), the medium was changed to HSM with dialyzed knockout serum replacement dialysis and without insulin, dexamethasone, or aprotinin. The 201B7 cells (arroheads) disappeared while the primary hepatocyes (arrows) were still alive at day 1 (B), day 2 (C) and day 3 (D). Original magnification: ×400, scale bar: 25 µm, arrow: 201B7 cells, arrowhead: hepatocytes.