Correlation between galectin-7 expression and NF-κB activity.

<p>(<b>A</b>) Putative binding sites for NF-κB, c-Rel and p53 within the proximal promoter region of the human <i>galectin-7</i> gene. The location of the putative binding sites were obtained by analyzing the sequence of the galectin-7 promoter using the TFSearch program which searches highly correlated sequence fragments against TFMATRIX transcription factor binding site profile database in the 'TRANSFAC' databases by GBF-Braunschweig (<u><a href="http://www.cbrc.jp/research/db/tfsearch.html" target="_blank">http://www.cbrc.jp/research/db/TFSEARCH.html</a></u>). (<b>B</b>) Galectin-7 protein levels measured by ELISA in MDA-MB-231, MDA-MB-453, MCF-7 and MDA-MB-468 breast cancer cell lines. Human epithelial keratinocytes (HaCaT) was used as a positive control. Similar results were obtained by Western blot analysis (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072468#pone.0072468.s004" target="_blank">Figure S4</a></b>). (<b>D</b>) NF-κB and p53 activity as measured following transient transfection with luciferase reporter vectors in breast cancer cell lines with different p53 status and HaCaT cells. Transfection efficiency was normalized with co-transfection with a β-galactosidase reporter vector.