Galectin-7 expression is dependent of NF-κB signalling pathway.

<p>mRNA levels following treatment with increasing doses of TNFα (<b>A</b>) or at different time post-treatment with TNFα (<b>B</b>). (<b>C</b>) <i>In silico</i> analysis showing effect of parthenolide on TNFα-induced <i>galectin-7</i> expression in epidermal keratinocytes. Data were retrieved from a dataset (GDS : 1289) in the Gene Expression Omnibus (GEO) repository. (<b>D</b>) Effect of parthenolide on galectin-7 expression in HaCaT cells. mRNA levels of <i>galectin-7</i> was measured by semi-quantitative RT-PCR analysis 48h after treatment. No such effect was observed on C/EBPβ expression. GAPDH was used as loading control. (<b>E</b>) Dose-dependent inhibition of <i>galectin-7</i> mRNA levels in MDA-MB-468 cells treated with CAPE. GAPDH was used as loading control. (<b>F</b>) Dose-dependent inhibition of <i>galectin-7</i> mRNA levels in MDA-MB-468 cells treated with quercetin. GAPDH was used as loading control.