Various treatments of neuroblastoma cells.

<p>(A), nutlin-3 treated NGP cells, 16 µM (right) and vehicle control (left). (B), ATRA treated NGP cells with elongated neurites (right) and vehicle control (left). (C), cell viability assay of IMR-32 and SK-N-SH cells after treatment with increasing concentrations of withaferin-A for 24 hours. Error bars, SD (n = 3). (D), cell viability assay after treatment of CLB-GA and SK-N-SH cells with increasing concentrations of TAE-684 for 24 hours. Error bars, SD (n = 3). (E), RT-qPCR expression data of PTK9 in SH-EP, SK-N-SH, SH-SY5Y and SK-N-BE(2c) after transfection with miR-1 mimic or scrambled miRNA mimic serving as a negative control (NC). Error bars, SEM (n = 2). (F), RT-qPCR expression data of T-UCR uc. 73 (left) and T-UCR uc.460 (right) 24 hours after transfection of SH-EP cells with siRNA against T-UCR uc. 73 and siRNA against T-UCR uc. 460 respectively. Errors bars, SEM (n = 2).