Thermal transition of EspB monitored by CD spectra at pH 7.0 in the presence or absence of GdnHCl.

<p>(A) The Far-UV CD spectra of EspB obtained at various temperatures in the absence of GdnHCl. Numbers refer to the temperature in °C. (B) Temperature dependences of the ellipticity at 222 nm ([<i>θ</i>]₂₂₂) of EspB in the absence (O) or presence of 2.0 (□), 3.0 (Δ), 4.0 (◊), 5.0 (∇), 6.0 (+) and 7.0 M (X) GdnHCl. Lines are the results of curve-fitting. (C) Linear dependence of Δ<i>G</i>_20°C against GdnHCl concentrations to estimate the Δ<i>G</i>_20°C in the absence of GdnHCl (Δ<i>G</i>_water) and <i>m</i> value from the slope of the plots. The Δ<i>G</i>_20°C obtained by the thermal transition curves of EspB and EspB41–70 are shown as circles and squares, respectively. (D) Temperature dependences of the ellipticity at 222 nm ([<i>θ</i>]₂₂₂) of EspB41–70 in the absence (O) or presence of 4.0 (◊), 5.0 (∇), 6.0 (+) and 7.0 M (X) GdnHCl. Lines are the results of curve-fitting.