Silencing of C9orf86 induces G1 arrest and apoptosis in breast cancer cells.

<p>(A) Cell cycle distribution was analyzed by flow cytometry 72 h after transfection. Bars are shown as the mean ± SD of cells in G1 phase of the cell cycle. (B) Apoptosis was determined by flow cytometric detection of Annexin-V-FITC-positive/PI-negative cells 72 h after infection. Bars are shown as the mean ± SD of cells with Annexin-V-FITC-positive and PI-negative. All data are shown as mean ± SD of two or three independent experiments, *P<0.05. NC, negative control.