Hypoxia induces basal lipolysis in human adipocytes independent of HBS.

<p>A. Hypoxia induces basal lipolysis in human adipocytes: Mature visceral (VAT) or subcutaneous (SAT) adipocytes were cultured in N, H conditions for 24 hrs, then glycerol release measured in supernatants with a spectrophotometric assay kit. Hypoxia induced basal lipolysis in visceral and subcutaneous adipocytes. Ordinate: glycerol concentration (mg/ml); asterisk: p<0.050, paired t-test, comparing N, H conditions; n = 10 obese subjects. B. Hypoxia does not regulate b-adrenergic stimulated lipolysis in VAT adipocytes: Mature VAT adipocytes were cultured in N, H conditions for 24 hrs with 3 mM isoproterenol then glycerol release measured in supernatants with a spectrophotometric assay kit. Hypoxia had no effect on isoproterenol-stimulated lipolysis in adipocytes. Ordinate: glycerol concentration (mg/ml); asterisk: p<0.050, paired t-test, comparing N, H conditions; n = 10 obese subjects. C. HBS does not regulate basal lipolysis in human adipocytes: Mature adipocytes were cultured in normoxic conditions for 24 hrs in the presence or absence of the HBS-inhibitor azaserine and supernatants studied for glycerol release. Ordinate: glycerol concentration (mg/ml); no statistically significant difference between media, azaserine arms for VAT or SAT adipocytes; n = 10 obese subjects.