<i>P. dolloi</i> gut epithelial cells (GEC) express J chain.

<p>A) P. <i>dolloi</i> gut cryosection stained with the nuclear stain DAPI (blue) showing the characteristic nuclear morphology of epithelial cells B) <i>P. dolloi</i> gut cryosection stained with Cy-3 J chain Stellaris FISH probe. Epithelial cells contained numerous J chain transcripts (dots). Ep: epithelial cells, Lu: lumen, Bm: basal membrane C) Gut epithelial cells were dissected from control <i>P. dolloi</i> gut cryosections using LCM. Total RNA from <i>P. dolloi</i> pre-pyloric spleen, LCM gut epithelial cells (GEC) and muscle was extracted and used as template for RT-PCR using J chain (J), IgM, IgW and cytokeratin 8 (CK-8) specific primers. EF-1α served as cDNA control. “–” indicates J chain negative control. M (marker) indicates the DNA ladder. The sequences of all PCR products were confirmed by cloning and sequencing.