Effects of peripheral nesfatin-1 infusion on GLUT4 in mice fed high fat diet.

<p>Immunofluorescent staining was performed using specific antibody against GLUT4 (red) in skeletal muscle and adipose tissue derived from mice consuming high fat diet and either saline (control) or nesfatin-1. Nuclei were stained with Hoechst dye. Controls include substituting primary antibodies with mouse IgG. Shown were representative results from six individual experiments. Images depict immunofluorescent staining for GLUT4 (red) in skeletal muscle (A) and adipose tissue (B). Data are expressed as mean±SEM as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071513#pone-0071513-g004" target="_blank">Figure 4</a>. *<i>P</i><0.05 vs. control, # <i>P</i><0.05 vs. insulin stimulation without nesfatin-1 treatment. Shown were representative results from six individual experiments.