Effects of peripheral nesfatin-1 infusion on GLUT 4 in NCD mice.

<p>(A, C) Immunofluorescent staining for GLUT4. Immunofluorescent staining was performed using specific antibody against GLUT4 (red) in skeletal muscle (A) and adipose tissue (C). Nuclei were stained with Hoechst dye. Controls include substituting primary antibodies with mouse IgG. Signal intensity of cytoplasm membrane GLUT4 immunoreactivity was quantified and normalized to the basal control, Data are expressed as mean±SEM. *<i>P</i><0.05 vs. control, # <i>P</i><0.05 vs. insulin stimulation without nesfatin-1 treatment. (B, D) Western blotting for GLUT4. Western blot was performed using specific antibody against GLUT4 in skeletal muscle (B) and adipose tissue (D). GAPDH was used as the internal control. Shown were representative results from six individual experiments.