Courtship parameters are unaffected in <i>NetAB</i> mutants but male behavior is altered.

<p>(<b>A</b>) Latency to initiate courtship (the time for the first courtship specific behavior to occur) is unaffected in <i>NetAB</i> mutants. All transgenic notation indicates one copy of each transgene. Data shown in bar graph are means ± s.e.m. (<b>B</b>) Courtship index (amount of time spent by the male courting) is unaffected in <i>NetAB</i> mutants. All transgenic notation indicates one copy of each transgene. Arcsine transformation was performed to satisfy assumptions of analysis of variance (not shown). Data shown in bar graph are means ± s.e.m. (<b>C</b>) <i>NetAB</i> mutants display mirror movements during courtship behavior. This phenotype was not rescued by any manipulation attempted, including endogenous <i>NetBmyc</i> expression in the <i>NetAB</i> mutant. All transgenic notation indicates one copy of each transgene (<i>w-not iso</i> compared to <i>NetAB</i>, p=0.012, Tukey HSD within a one-way ANOVA). Arcsine transformation was performed on percentage of mirror movements to satisfy assumptions of analysis of variance (not shown). Data shown in bar graph are means ± s.e.m. n ≥ 9 in all genotypes except NetAB; UASptenRNAi, n=4 and 938 NetAB, n=5.