Electron microscopy of lipid droplets in sporulating cells.

<p>Electron micrographs of wild type cells. A) A cell in Meiosis II showing two prospore membranes (green arrows) engulfing nuclear lobes (N). Lipid droplets can be seen associated with the prospore membrane (yellow arrowheads). Scale bar = 500 nm. B) Higher magnification of one of the prospore membranes in A. Scale bar = 100 nm. C) Post meiotic cell showing dark-stained lipid droplets (yellow arrowhead) associated with the outer membrane (green arrow). Lipid droplets inside of the spore (yellow arrow) do not stain as darkly with permanganate. N = nucleus. Scale bar = 500 nm. D) Lipid droplets in the ascus (yellow arrowhead) associated with the outer spore wall (red arrow) of mature spores. Yellow arrow indicated a lipid droplet within the spore cytoplasm. N = nucleus. Scale bar = 500 nm.