Multiple sequence alignment (MSA), secondary and tertiary structures of four selected Plk1 specific substrates.

<p>(a) SMARCAD1, (b) GSG2, (c) NUP35, (d) NEK5, (e) KIF23 and (f) CEP170. MSA illustrates the conservation pattern of predicted PBD recognition and kinase phosphorylation motifs across close homologues of selected proteins (a–d). Secondary structure for each substrate was predicted by JPred as shown below the alignments Tertiary structures of (a–f) substrates are shown in cornflower blue ribbon-form, phosphorylation motifs are shown in yellow, PBD recognition motifs are shown in pink.