Effect of human SMN2 expression on proliferation and snRNP assembly in Smn-deficient NIH3T3 cell lines.

<p>(A) Analysis of cell proliferation in wild-type (Control), NIH3T3-Smn_RNAi, NIH3T3-SMN2_low/Smn_RNAi and NIH3T3-SMN2_high/Smn_RNAi cell lines cultured with and without doxycycline for 7 days. For each cell line, the cell number ratio of Dox-treated cells versus untreated cells is expressed relative to that of wild-type cells, which is set to 1. Data are represented as mean and SEM (n≥3; *** = p<0.001; one-way ANOVA). (B) Analysis of U1 snRNP assembly in NIH3T3 cell lines. <i>In vitro</i> snRNP assembly experiments were carried out with radioactive U1 snRNA and extracts from wild-type NIH3T3 (Control), NIH3T3-Smn_RNAi, NIH3T3-SMN2_low/Smn_RNAi and SMN2_high/Smn_RNAi cells cultured with and without doxycycline for 7 days. For each cell line, the amounts of immunoprecipitated U1 snRNA were quantified and the RNA ratio in Dox-treated cells versus untreated cells is expressed relative to that of wild-type cells, which is set to 1. Data are represented as mean and SEM (n≥3; * = p<0.05; ** = p<0.01; *** = p<0.001; one-way ANOVA).