Binding of Sfl1p-HA₃ and Sfl2p-HA₃ to selected target promoters.

<p>Strains <i>sfl1</i>-CaEXP-<i>SFL1-HA₃</i> (Sfl1p-HA₃) and <i>sfl2</i>-CaEXP-<i>SFL2-HA₃</i> (Sfl2p-HA₃) together with their respective untagged control strains (Vector) were grown under the same conditions as those for the ChIP-Seq experiment prior to ChIP followed by PCR to detect specific Sfl1p and Sfl2p binding enrichment at selected target promoters (See <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003519#s4" target="_blank">Materials and Methods</a> for details). PCR was performed using primers corresponding to the promoter region of the indicated genes. The <i>URA3</i> and <i>YAK1</i> genes were used as a negative control for ChIP enrichment. Primer efficiency (shown on the right panel) was tested by the ability of the corresponding primers to quantify 10-fold serially diluted whole cell extract DNA (WCE, ChIP input samples, dilution factors are indicated at the top of the right panel).