Eight TM4 deafness mutations cause mis-trafficking.

<p>All 14 mutations were tested for their ability to make gap junctions in transiently transfected HeLa cells. Eight mutations (K188R, F191L, V198M, S199F, G200R, I203K, L205P, T208P) caused mis-trafficking, typically with intracellular aggregation. WT human Cx26 is shown at the top left for comparison, with an arrow pointing to a gap junction. The arrowhead in the F191L image points to a cell-cell apposition area with aggregate fluorescence.