Myoblast differentiation is induced by EGFR knockdown.

<p>Proliferating myoblasts were transfected either with a control siRNA or with a siEGFR, and then maintained in proliferation condition for 5 days. <b>A.</b> Total cell lysates were analyzed by Western Blot. Myogenin and MEF2 expressions were used as early markers of differentiation, Myosin heavy chain (MyHC) as a late marker of differentiation, and α-Tubulin as a loading control. <b>B</b>. Differentiation of myoblasts was observed by the staining of MEF2 (red) and MyHC (green), and nuclei by DAPI (blue). Cesium (Cs 10 mM) was added to the medium to block the hyperpolarization. Myotubes resulting from myoblast fusion were observed (white arrows). <b>C.</b> Quantification of the immuno-fluorescence shown in B (n>3).