Breakpoint sequences of reciprocal duplication/deletion alleles <i>P1-rr-T1</i> and <i>p1-ww-T1</i> generated by Reversed Ends Transposition.

<p>(<i>A</i>) Diagram shows the structure of the progenitor <i>P1-ovov454</i> allele prior to RET. Two sister chromatids are shown, with symbols as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003691#pgen-1003691-g001" target="_blank">Figure 1</a>. The dotted box shows the a/b target site region, whose sequence is indicated above. The color of the letters in the sequences matches the chromatid line color. (<i>B</i>) PCR amplification of the deletion/duplication breakpoints in <i>p1-ww-T1</i> and <i>P1-rr-T1</i> with primers 2+Ac3 (upper panel) or 1+Ac5 (lower panel). Lane 1: DNA ladder, lane 2: <i>p1-ww[4Co63]</i>, lane 3: <i>P1-ovov454/p1-ww[4Co63]</i>, lane 4: <i>p1-ww-T1/p1-ww[4Co63]</i>, lane 5: <i>P1-rr-T1/p1-ww[4Co63]</i>. (<i>C</i>) Sister chromatid structures of <i>p1-ww-T1</i> (upper) and <i>P1-rr-T1</i> (lower). Sequences of the deletion and duplication breakpoints (dotted boxes) are shown in color matching the chromatid line color. Note that each breakpoint has a copy of the 8 bp TSD GCGCTTTA which is present in a single copy at the a/b target site in the progenitor allele.