Swiprosin-1 mediates cell spreading and migration.

<p>(A) HeLa cells were transfected with scrambled siRNA or siRNA targeting <i>swiprosin-1</i> for 48 h, and the efficiency of siRNA transfection was then determined by RT-PCR (A–a, top) and Western blotting (A–a, bottom). The cells were plated on FN-coated coverslips for 30 and 60 min. The average cell area (A–b) and percentage of fully spread cell (cell area >1500 µm²) (A–c) were then determined. (A–d) F-actin was stained with phalloidin-Alexa 488, and the fluorescence signals were analyzed by confocal microscopy. Scale bars: 20 µm. (B) The cells from (A) were subjected to a wound-healing assay. The average rates of wound closure were determined 10 h after the wounding (B–a) and visualized by phase contrast microscopy (B–b). The results are expressed as the mean ± SD of triplicate experiments. Sc, scrambled. <i>*P</i><0.05 <i>vs.</i> scrambled siRNA-transfected cells.