Quantification of residual γ-H2AX 24 h after radiation with different doses.

<p>A549 and MCF-7 cells were pretreated with or without 700 nM nimotuzumab for 24 h, and then were radiated with 1, 2, 4 and 8 Gy. Twenty four hours after radiation cells were fixed and incubated with γ-H2AX antibody conjugated with FITC. Each bar represents the mean ± SD of residual γ-H2AX foci per cell. For each data point at least 300 nuclei were evaluated. * indicates significant difference (P<0.05). The images below are representative nuclei with γ-H2AX foci under immunofluorescent microscopy, treated with 1Gy or 1Gy combined with nimotuzumab.