Reduced BDNF-dependent activation of TrkB in SORLA-deficient neurons.

<p>(<b>A, B</b>) Quantification of phosphorylated (p) forms of TrkB, Akt, and ERK in primary cortical neurons either non-treated (−) or treated with 150 ng/ml BDNF for 20 min (+) using Western blotting (A) and densitometric scanning of replicate blots (B). <i>Sorl1^−/−</i> neurons show reduced levels of pTrkB, pAKT, and pERK compared with <i>Sorl1^+/+</i> cells (n = 6–12, Mann-Whitney U test). (<b>C, D</b>) Quantification of total levels of TrkB, Akt, and ERK in primary neurons either non-treated (−) or treated with 150 ng/ml BDNF (+) for 20 min using Western blot (C) and densitometric scanning of replicate blots (D). Detection of tubulin (tub.) served as loading controls in A and C.