Cytokines production is lower in PARP-1^−/− T cells.

<p>(<b>A</b>) The profile of indicated cytokines in the supernatants of PARP-1^−/− (black bar) and control WT (white bar) whole spleen cells stimulated with soluble anti-CD3-antibody (0.5 µg/ml) for one (IL-2, IL-4), two (TNF) and three (others) days. (<b>B</b>) Cytokine production in purified CD4⁺CD25⁻ T cells stimulated with plate-coated anti-CD3 (5 µg/ml) for the same time as A. The cytokines were determined with ELISA assay. The data are shown as Mean±SD of duplicate measurements and representative of 3–4 experiments, **p<0.01, *p<0.05.