Further defects in chemoattractant signaling in the <i>Gnai2</i>^fl/fl<i>mb1-cre</i> and DKO mice.

<p>A. Changes in intracellular calcium were monitored over 3 minutes in B cells from control and Gnai2 ^fl/fl<i>mb1-cre</i> mice exposed to increasing concentrations of CXCL12. The data shown as fluorescent counts and the y-axis labeled as iLm1. Each experimental value is the mean of three determinations. Similar results using B cells prepared from 3 mice of each genotype. B. Changes in intracellular calcium were monitored over 3 minutes using B cells from control and mutant mice exposed to CXCL12 or CXCL13. The data shown as fluorescent counts and the y-axis labeled as iLm1. Data from B cells prepared from 2 mice of each genotype. C. <i>In vivo</i> homing assay using B cells from control and mutant mice. B cells from each genotype were fluorescently labeled and adoptively transferred to a wild type recipient. The cells in the blood, spleen, inguinal and axillary LNs (pLN), and the mesenteric LNs were enumerated by flow cytometry. Data shown as mean+/- SEM. Data analyzed by <i>t</i> test. D. Images from multiphoton microscopy of the spleen and inguinal LN following the adoptive transfer of control and DKO B cells. Fluorescently labeled <i>Gnai2</i>^fl/fl B cells were adoptively transferred 5 h (blue) and 24 h (red) prior to imaging. The DKO B cells (green) were transferred 5 h prior to imaging. The LN was imaged intravitally while a fresh section was used for the spleen imaging. Evans blue was infused intravenously immediately before the imaging to outline blood vessels. ** p<0.001, * p<0.01.