Evaluation of migration rates of non-differentiated THP-1 cells and PMA differentiated macrophages.

<p>The migration rate was higher when macrophages were exposed to benzalkonium chloride (BAK) (16.2±2.1%, <i>P</i> = 0.001,*), lipopolysaccharide (LPS) (15.1%±4.7%, <i>P</i> = 0.004, §) or tumor necrosis factor alpha (TNF-α) (29.9±4.7%, <i>P</i><0.0001, #) as compared to macrophages exposed to phosphate buffered saline (PBS). Migration was significantly higher when macrophages were exposed to TNF-α as compared to BAK (<i>P</i> = 0.004). Migration was measured in coculture with conjunctival cells except for column 2. Errors bars represent standard deviation.