Characterizations of LT-induced hypoploid cells.

<p>Human cord blood-derived CD34⁺ cells were treated using LT and rLT on Day 12 and analyzed on Day 16 during the <i>in vitro</i> differentiation of erythrocytes. The DNA contents were revealed by fluorescence intensities of propidium iodine (PI) staining. The hypoploid cells were increased in LT-treated and rLT-treated groups (A). Annexin V-APC (B) and active caspase-3-FITC antibodies (C) were used to characterize the apoptotic cells in R2 region by flow cytometry. The percentage of sub-G1 cells on day 0 and annexin V⁺ and active caspase-3⁺ cells of R1 region on day 0 were used as basal level controls. Representative histograms (A–C) and summarized events (D) were shown. *<i>p</i><0.05, ** <i>p</i><0.01, compared to vehicle-treated controls. Data are reported as mean ± SD and represent results from 3 independent experiments.