Differentiation protocol and changes in cell morphology during neural progenitor induction.

<p>Light microscopy images show the differentiation process of hiPSC line 8 (A,a) into neural ectoderm (NE; B,b), rosette formation (C,c) and neural tube-like structure (NT; D,d) over three weeks. To expand neural progenitors (NPs), the NT structures were mechanically isolated using an inverted phase contrast microscope at day 18 and replated on poly-l-ornithine/laminin-coated plates (E), then passaged every 4–6 days. (F) The morphology of cells at passage 6.