Rapamycin (RAPA) can induce autophagy.

<p>After the injection of rapamycin and vecicle 30 min before trauma, the rats were sacrificed at 6 h after trauma. (A and B) The protein and mRNA levels of Beclin 1 and LC3 were detected. Data are expressed as Mean±SD (n = 6). * p<0.05; ** p<0.01. (C) Confocal images of Beclin 1 and LC3. Beclin 1 and LC3 immunoreactivity were present as punctate pot (red) and the nucleus were stained with DAPI (blue). Compared with control-operated animals, trauma group showed decreased staining of Beclin 1 and LC3 6 h after injury. Treatment with rapamycin (RAPA) partially reversed nonlethal trauma-induced down-regulation of staining.